解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The army of the men of death, in John Bunyan's memorable phrase, has a new recruit, and fear has a new face: a face wearing a surgical mask. ...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-7-113
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Subcellular messenger RNA localization is important in most eukaryotic cells, even in unicellular organisms like yeast for which this process has been underestimated. Microarrays are rarely used to study subcellular mRNA localization at whole-genome level, but can be adapted to that purpose. This work focuse...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-7-r44
更新日期:2003-01-01 00:00:00
abstract::Protein kinases with a conserved catalytic domain make up one of the largest 'superfamilies' of eukaryotic proteins and play many key roles in biology and disease. Efforts to identify and classify all the members of the eukaryotic protein kinase superfamily have recently culminated in the mining of essentially complet...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-5-111
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:A large fraction of the human genome is attributable to L1 retrotransposon sequences. Not only do L1s themselves make up a significant portion of the genome, but L1-encoded proteins are thought to be responsible for the transposition of other repetitive elements and processed pseudogenes. In addition, L1s ca...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-5-r30
更新日期:2003-01-01 00:00:00
abstract::Microarrays have been used in a wide variety of experimental systems, but realizing their full potential is contingent on sophisticated and rigorous experimental design and data analysis. This article highlights what is needed to get the most out of microarrays in terms of accurately and effectively revealing differen...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-2-105
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Candida glabrata is a pathogenic yeast of increasing medical concern. It has been regarded as asexual since it was first described in 1917, yet phylogenetic analyses have revealed that it is more closely related to sexual yeasts than other Candida species. We show here that the C. glabrata genome contains ma...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2003-4-2-r10
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The H subunit of the purple bacterial photosynthetic reaction center (PRC-H) is important for the assembly of the photosynthetic reaction center and appears to regulate electron transfer during the reduction of the secondary quinone. It contains a distinct cytoplasmic beta-barrel domain whose fold has no clo...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-11-research0061
更新日期:2002-10-14 00:00:00
abstract:BACKGROUND:As the rough draft of the human genome sequence nears a finished product and other genome-sequencing projects accumulate sequence data exponentially, bioinformatics is emerging as an important tool for studies of transposon biology. In particular, L1 elements exhibit a variety of sequence structures after in...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-10-research0052
更新日期:2002-09-19 00:00:00
abstract::How do naturally occurring polymorphisms in DNA sequence relate to variation in gene expression? Recent work to map genetic sources of expression variation has shown a surprising balance between cis and trans effects. Other work suggests some chromosomal clustering of genes by expression pattern. A synthesis of approa...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-10-reviews1029
更新日期:2002-09-19 00:00:00
abstract:BACKGROUND:Complete genomic sequences of closely related organisms, such as the chlamydiae, afford the opportunity to assess significant strain differences against a background of many shared characteristics. The chlamydiae are ubiquitous intracellular parasites that are important pathogens of humans and other organism...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-9-research0051
更新日期:2002-08-29 00:00:00
abstract::A report on the Genome-Based Pathogen Biology meeting, Hinxton, UK, 7-10 July 2002. ...
journal_title:Genome biology
pub_type:
doi:10.1186/gb-2002-3-9-reports4029
更新日期:2002-08-27 00:00:00
abstract::DNA shuffling has proven to be a powerful technique for the directed evolution of proteins. A mix of theoretical and applied research has now provided insights into how recombination can be guided to more efficiently generate proteins and even organisms with altered functions. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-8-reviews1021
更新日期:2002-07-31 00:00:00
abstract::A report on the Cold Spring Harbor Laboratory meeting on the Evolution of Developmental Diversity, Cold Spring Harbor, NY, USA, 17-21 April 2002. ...
journal_title:Genome biology
pub_type:
doi:10.1186/gb-2002-3-8-reports4023
更新日期:2002-07-15 00:00:00
abstract:BACKGROUND:Microarray technology is increasingly being applied in biological and medical research to address a wide range of problems, such as the classification of tumors. An important statistical problem associated with tumor classification is the identification of new tumor classes using gene-expression profiles. Tw...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-7-research0036
更新日期:2002-06-25 00:00:00
abstract::We used scaled factorial moments to search for intermittency in the log expression ratios (LERs) for thousands of genes spotted on cDNA microarrays (gene chips). Results indicate varying levels of intermittency in gene expression. The observation of intermittency in the data analyzed provides a complimentary handle on...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-7-preprint0005
更新日期:2002-05-29 00:00:00
abstract:BACKGROUND:Gene duplications have a major role in the evolution of new biological functions. Theoretical studies often assume that a duplication per se is selectively neutral and that, following a duplication, one of the gene copies is freed from purifying (stabilizing) selection, which creates the potential for evolut...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-2-research0008
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:High-density oligonucleotide microarrays provide a powerful tool for assessing differential mRNA expression levels. Characterizing the noise resulting from the enzymatic and hybridization steps, called type I noise, is essential for attributing significance measures to the differential expression scores. We ...
journal_title:Genome biology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The use of DNA sequences to estimate the timing of evolutionary events is increasingly popular, although it is fraught with practical difficulties. But the exponential growth of relevant information and improved methods of analysis are providing increasingly reliable sequence-derived dates, and it may become possible ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-3-1-reviews0001
更新日期:2002-01-01 00:00:00
abstract::Big projects in biology - such as the human genome project and a number of related and ensuing enterprises - require big funding. A new tradition is growing in which some types of basic research take place within commercial organizations. This article reviews some of the reasons for this and some of the key players, i...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-4-comment2004
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:In microarray data analysis, the comparison of gene-expression profiles with respect to different conditions and the selection of biologically interesting genes are crucial tasks. Multivariate statistical methods have been applied to analyze these large datasets. Less work has been published concerning the a...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-4-research0019
更新日期:2002-01-01 00:00:00
abstract::Genetic mapping and determination of the organization of the wheat genome are changing the wheat-breeding process. New initiatives to analyze the expressed portion of the wheat genome and structural analysis of the genomes of Arabidopsis and rice are increasing our knowledge of the genes that are linked to key agronom...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-5-reviews1013
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Concepts of orthology and paralogy are become increasingly important as whole-genome comparison allows their identification in complete genomes. Functional specificity of proteins is assumed to be conserved among orthologs and is different among paralogs. We used this assumption to identify residues which de...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-3-preprint0002
更新日期:2002-01-01 00:00:00
abstract::A recent study has used serial analysis of gene expression to compare mouse forelimb and hindlimb gene-expression profiles. The method successfully identified known regulators of limb identity and has generated a candidate set of differentially expressed genes that may regulate limb identity. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-3-reviews1007
更新日期:2002-01-01 00:00:00
abstract::Two recent studies have added microarrays to the toolkit used to analyze the origins of replication in yeast chromosomes, providing a fuller picture of how genomic DNA replication is organized. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-6-reviews1016
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND:Methods of microarray analysis that suit experimentalists using the technology are vital. Many methodologies discard the quantitative results inherent in cDNA microarray comparisons or cannot be flexibly applied to multifactorial experimental design. Here we present a flexible, quantitative Bayesian framewor...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2002-3-12-research0071
更新日期:2002-01-01 00:00:00
abstract::We describe here our experience in annotating the Drosophila melanogaster genome sequence, in the course of which we developed several new open-source software tools and a database schema to support large-scale genome annotation. We have developed these into an integrated and reusable software system for whole-genome ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-12-research0081
更新日期:2002-01-01 00:00:00
abstract::Repeated DNA elements and region-specific protein modifications combine within chromosomes to form a transcriptionally silent chromatin structure called heterochromatin. Recent work in the fission yeast Schizosaccharomyces pombe reveals that RNA is also an integral component of silent heterochromatin, providing a new ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2002-3-12-reviews1035
更新日期:2002-01-01 00:00:00
abstract:SUMMARY:The large transforming growth factor-beta (TGFbeta) superfamily of secreted proteins regulate the growth, development and differentiation of cells in diverse organisms, including nematode worms, flies, mice and humans. Signals are initiated upon binding of TGFbeta superfamily members to cell-surface serine/thre...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-8-reviews3010
更新日期:2001-01-01 00:00:00
abstract::In most eukaryotes, histones, which are the major structural components of chromatin, are expressed as a family of sequence variants encoded by multiple genes. Because different histone variants can contribute to a distinct or unique nucleosomal architecture, this heterogeneity can be exploited to regulate a wide rang...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-7-reviews0006
更新日期:2001-01-01 00:00:00
abstract::Gene-expression profiling has yielded important information about simple systems, but complex tissues have not yet been widely profiled. Four recent studies of mammalian skeletal muscles have added to the catalogs of their gene expression differences, but have yet to lead to better understanding of the molecular proce...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-12-reviews1033
更新日期:2001-01-01 00:00:00
abstract::Ever since DNA microarrays were first applied to the quantitation of RNA levels, there has been considerable interest in generating a protein homolog that can be used to assay cellular protein expression. A recent paper describes the first microarray that can be used for such protein profiling. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-2-reviews1004
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Despite decades of research, the agent responsible for transmitting spongiform encephalopathies (TSEs) has not been identified. The Prion hypothesis, which dominates the field, supposes that modified host PrP protein, termed PrPSc, acts as the transmissible agent. This model fits the observation that TSE dis...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-7-preprint0006
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:We have constructed Bayesian prior-based, amino-acid sequence profiles for the complete yeast mitochondrial proteome and used them to develop methods for identifying and characterizing the context of protein mutations that give rise to human mitochondrial diseases. (Bayesian priors are conditional probabilit...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-6-research0021
更新日期:2001-01-01 00:00:00
abstract::Completion of fungal, plant and human genomes paved the way to the identification of erythrocytic rhesus proteins and their kidney homologs as ammonium transporters. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-3-reviews1010
更新日期:2001-01-01 00:00:00
abstract::A report on the 18th Congress of the European Society for Evolutionary Biology (ESEB), Aarhus, Denmark, 20-25 August, 2001. ...
journal_title:Genome biology
pub_type:
doi:10.1186/gb-2001-2-11-reports4026
更新日期:2001-01-01 00:00:00
abstract::Epithelial tissues house gammadelta T cells, which are important for the mucosal immune system and may be involved in controlling malignancies, infections and inflammation. Whole-genome gene-expression analysis provides a new way to study the signals required for the activation of gammadelta T cells, their mode of act...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-11-reviews1031
更新日期:2001-01-01 00:00:00
abstract::Recent experiments show that properly controlled recombination between homologous DNA molecules is essential for the maintenance of genome stability and for the prevention of tumorigenesis. ...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-5-reviews1014
更新日期:2001-01-01 00:00:00
abstract::Nuclear pore complexes, the conduits for information exchange between the nucleus and cytoplasm, appear broadly similar in eukaryotes from yeast to human. Precisely how nuclear pore complexes regulate macromolecular and ionic traffic remains unknown, but recent advances in the identification and characterization of co...
journal_title:Genome biology
pub_type: 杂志文章,评审
doi:10.1186/gb-2001-2-9-reviews0007
更新日期:2001-01-01 00:00:00
abstract::Searching for similarities between biological sequences is the principal means by which bioinformatics contributes to our understanding of biology. Of the various informatics tools developed to accomplish this task, the most widely used is BLAST, the basic local alignment search tool. This article discusses the princi...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-10-reviews2002
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:We propose a new method for supervised learning from gene expression data. We call it 'tree harvesting'. This technique starts with a hierarchical clustering of genes, then models the outcome variable as a sum of the average expression profiles of chosen clusters and their products. It can be applied to many...
journal_title:Genome biology
pub_type: 杂志文章
doi:10.1186/gb-2001-2-1-research0003
更新日期:2001-01-01 00:00:00